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BS combined with DAC showed enhanced effects in inhibiting the survival of lung cancer cells. A549 and H1299 cells were treated with BS, DAC, or BS + DAC (3:1 for A549 [BS-5 μM, DAC-1.67 μM] and 2:1 for H1299 [BS-5 μM, DAC-2.5 μM]) for 48 h, followed by Annexin V-PI staining. The results of total apoptosis (including both early and late apoptotic populations) are shown as mean ± SD. * P < 0.05, *** P < 0.001, **** P < 0.0001, compared with each indicated group by one-way ANOVA followed by Tukey’s post hoc test analysis ( n = 3).

Journal: Scientific Reports

Article Title: The combined inhibitory effect of butaselen and decitabine against lung cancer cells

doi: 10.1038/s41598-026-45054-7

Figure Lengend Snippet: BS combined with DAC showed enhanced effects in inhibiting the survival of lung cancer cells. A549 and H1299 cells were treated with BS, DAC, or BS + DAC (3:1 for A549 [BS-5 μM, DAC-1.67 μM] and 2:1 for H1299 [BS-5 μM, DAC-2.5 μM]) for 48 h, followed by Annexin V-PI staining. The results of total apoptosis (including both early and late apoptotic populations) are shown as mean ± SD. * P < 0.05, *** P < 0.001, **** P < 0.0001, compared with each indicated group by one-way ANOVA followed by Tukey’s post hoc test analysis ( n = 3).

Article Snippet: The cells were collected, and the apoptosis assay was conducted using the Annexin V-FITC/ propidium iodide (PI) Dual Staining Kit (KeyGEN) as instructed by the manufacturer and described previously .

Techniques: Staining

Functional consequences of MTHFD2 knockdown across apoptotic, migratory, and invasive phenotypes in OSCC. A , B Apoptotic incidence was assessed using Annexin V/PI dual-labeling cytometry in OSCC cells with stable MTHFD2 knockdown compared to control shRNA. C , D Cell invasion capacity under MTHFD2 knockdown was evaluated using a Transwell assay, with representative fields and cell penetration indices quantified. E–F Cell migratory capacity under MTHFD2 knockdown was assessed using scratch-wound closure kinetics, along with representative images and quantification of migrated OSCC cells. ** P < 0.01, *** P < 0.001

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: Investigating the oncogenic potential of MTHFD2 in oral squamous cell carcinoma

doi: 10.1007/s00432-026-06451-7

Figure Lengend Snippet: Functional consequences of MTHFD2 knockdown across apoptotic, migratory, and invasive phenotypes in OSCC. A , B Apoptotic incidence was assessed using Annexin V/PI dual-labeling cytometry in OSCC cells with stable MTHFD2 knockdown compared to control shRNA. C , D Cell invasion capacity under MTHFD2 knockdown was evaluated using a Transwell assay, with representative fields and cell penetration indices quantified. E–F Cell migratory capacity under MTHFD2 knockdown was assessed using scratch-wound closure kinetics, along with representative images and quantification of migrated OSCC cells. ** P < 0.01, *** P < 0.001

Article Snippet: Apoptosis in OSCC cells (shMTHFD2/shNC-transfected) was evaluated using an annexin V-fluorescein isothiocyanate/propidium iodide (PI) dual-staining kit (KGA108; KeyGEN, China).

Techniques: Functional Assay, Knockdown, Labeling, Cytometry, Control, shRNA, Transwell Assay